Combinatory RNA-Sequencing Analyses Reveal a Dual Mode of Gene Regulation by ADAR1 in Gastric Cancer

• DOI: 10.1007/s10620-018-5081-9
• Publication Date: 2018-04-25T01:55:46Z
• Authors: Charles J. Cho; Jaeeun Jung; Lushang Jiang; Eun Ji Lee; Dae-Soo Kim; Byung Sik Kim; Hee Sung Kim; Hwoon-Yong Jung; Ho-June Song; Sung Wook Hwang; Yangsoon Park; Min Kyo Jung; Chan Gi Pack; Seung-Jae Myung; Suhwan Chang
• Keywords: 0301 basic medicine; Binding Sites; Adenosine Deaminase; Sequence Analysis, RNA; Computational Biology; RNA-Binding Proteins; Introns; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Alu Elements; Stomach Neoplasms; Cell Line, Tumor; Humans; RNA Editing; RNA, Messenger; 3' Untranslated Regions

Adenosine deaminase acting on RNA 1 (ADAR1) is known to mediate deamination of adenosine-to-inosine through binding to double-stranded RNA, the phenomenon known as RNA editing. Currently, the function of ADAR1 in gastric cancer is unclear.This study was aimed at investigating RNA editing-dependent and editing-independent functions of ADAR1 in gastric cancer, especially focusing on its influence on editing of 3' untranslated regions (UTRs) and subsequent changes in expression of messenger RNAs (mRNAs) as well as microRNAs (miRNAs).RNA-sequencing and small RNA-sequencing were performed on AGS and MKN-45 cells with a stable ADAR1 knockdown. Changed frequencies of editing and mRNA and miRNA expression were then identified by bioinformatic analyses. Targets of RNA editing were further validated in patients' samples.In the Alu region of both gastric cell lines, editing was most commonly of the A-to-I type in 3'-UTR or intron. mRNA and protein levels of PHACTR4 increased in ADAR1 knockdown cells, because of the loss of seed sequences in 3'-UTR of PHACTR4 mRNA that are required for miRNA-196a-3p binding. Immunohistochemical analyses of tumor and paired normal samples from 16 gastric cancer patients showed that ADAR1 expression was higher in tumors than in normal tissues and inversely correlated with PHACTR4 staining. On the other hand, decreased miRNA-148a-3p expression in ADAR1 knockdown cells led to increased mRNA and protein expression of NFYA, demonstrating ADAR1's editing-independent function.ADAR1 regulates post-transcriptional gene expression in gastric cancer through both RNA editing-dependent and editing-independent mechanisms.