Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer

• DOI: 10.1007/s10620-024-08536-0
• Publication Date: 2024-07-17T19:02:01Z
• Authors: Chun-Wei Tang; Jin-Hua Yang; Jing-Wen Qin; Hui-Jie Wu; Hao-Peng Cui; Lian-Ying Ge; Ai-qun Liu
• Keywords: Transplant surgery

NK cells play a vital role in tumor immune resistance. Various factors affect cell activity. While dysfunction has been observed numerous malignancies, the underlying mechanisms gastric cancer remain unclear. Flow cytometry was used to identify phenotypic distribution and expression of activated receptors on cells. ELISA determine cytokines. We examined cell-related genes explored their association with survival prognosis. Additionally, we conducted PCR detection miR-552-5p levels plasma exosomes patients investigated its correlation receptors. flow verify dysfunction. Furthermore, potential PD-1/PD-L1 regulating patients' significant decrease percentage NKG2D NKp30 IFN-γ TNF-α than healthy volunteers. Patients low CD56, CD16, NKG2D, NKP46 exhibited poorer prognoses. Moreover, increased exosomal were negatively associated receptor expression. MiR-552-5p downregulated secretion perforin, granzyme, as well NKp30, NKp46, NKG2D. it suppressed cytotoxicity The inhibitory effect miR-552-5p, function reversed when anti-PD-L1 antibodies used. Exosomal targets axis, leading impaired function.