Morphological and molecular characterisation of Paralongidorus rex Andrássy, 1986 (Nematoda: Longidoridae) from Poland and Ukraine

• DOI: 10.1007/s10658-014-0550-2
• Publication Date: 2014-10-16T09:11:36Z
• Authors: Franciszek Wojcie...; Solomija Susulovska; Andrij Susulovsky; Sergei A. Subbotin
• Keywords: Morphometrics; 28S ribosomal RNA

Paralongidorus rex was found for the first time in Poland and Ukraine. This paper describes females juveniles from four populations of this species on basis morphology morphometrics provides molecular characterization using 18S, ITS1 D2-D3 expansion segments 28S rRNA gene sequences. Morphometrically, these differed slighty V ratio (means populations: 41.9; 42.7; 46.1; 46.8) odontostylet length (166.6; 170.6; 191.5; 193.2). Phylogenetic analysis showed that P. had a sister relationship with iranicus. PCR-D2-D3 28S-RFLP diagnostic profiles five enzymes are given. Additionally, information new host plants map distribution provided. The record nematode species, previously identified as sp. (GenBank: AY601582) Slovakia, is defined based comparison sequences gene. Finally, remarks potential importance grapevine production