Effects of a probiotic, protexin, on the growth performance and hematological parameters in the Oscar (Astronotus ocellatus) fingerlings

• DOI: 10.1007/s10695-011-9481-4
• Publication Date: 2011-04-12T09:58:38Z
• Authors: F. Firouzbakhsh; F. Noori; M. K. Khalesi; K. Jani-Khalili
• Keywords: 0301 basic medicine; Leukocyte Count; Random Allocation; 03 medical and health sciences; Probiotics; Erythrocyte Count; Animals; Aquaculture; Perciformes; 3. Good health

This study examined the effects of a probiotic, protexin, on the growth performance and hematological parameters in an ornamental fish, the Oscar Astronotus ocellatus fingerlings. A completely randomized experimental design was applied with three experimental diets each with three replicates. A commercial food, BioMar, was supplemented with protexin at levels of 0.15, 0.5, and 1.0 g kg(-1) dry food and fed three times a day for 60 days. The control diet was prepared with no protexin supplementation. The experimental fish were biometried every 15 days to compare their growth rates at each treatment. For hematological assays, blood samples were prepared every 30 days to measure such parameters as red and white blood cells, hemoglobin, hematocrit, and percentages of lymphocytes, monocytes, neutrophiles, basophiles, and eosinophiles. Based on the results, the fingerlings fed a 0.15 g kg(-1) supplemented food were significantly different from the fish in the other treatments and in the control, with the highest mean of both final weight (35.07 ± 1.19) and body weight gain (30.17 ± 1.08). Significant differences in both hemoglobin concentration and mean red and white blood cells were found between the experimental groups and the control within 2 months. The highest hemoglobin concentration and also red and white blood cells was observed in the fish-fed 0.15 dietary protexin in both months. The results of this study show that the probiotic, protexin, at a level of 0.15 g kg(-1) dry food could have measurable effects on the growth and hematological parameters in the Oscar A. ocellatus fingerlings.