Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system

• DOI: 10.1007/s10719-017-9772-9
• Publication Date: 2017-04-28T10:20:21Z
• Authors: Ken Kusama; Yuki Okamoto; Keiko Saito; Tsukasa Kasahara; Teizo Murata; Yasushi Ueno; Yuka Kobayashi; Yoshihiro Kamada; Eiji Miyoshi
• Keywords: Haptoglobin; Fucosylation

An increase in Lewis- and core-type fucosylation of haptoglobin has been reported patients with pancreatic cancer (PC), suggesting that fucosylated is a candidate PC biomarker. Previously, we developed Pholiota squarrosa lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection core-fucosylated haptoglobin. However, this methodology, positive results were only obtained some PC, demonstrating need more sensitive system. In current study, an improved PhoSL-ELISA higher sensitivity to detect using high-concentration urea as denaturing agent facilitate detection. We then reevaluated performance PhoSL reactive-core-fucosylated (PhoSL-HP) biomarker PhoSL-HP levels sera significantly than those healthy volunteers, area under curve (AUC) value 0.753. Furthermore, AUC CA19–9 from 0.793 0.907 when combined PhoSL-HP. Additionally, several CA19–9-negative cases among diagnosed conclusion, our ELISA might allow serve potential thus be useful complement diagnosis.