Subcellular localization of SARS-CoV-2 E and 3a proteins along the secretory pathway

• DOI: 10.1007/s10735-025-10375-w
• Publication Date: 2025-03-01T04:05:01Z
• Authors: Joshua J. Hinkle; Kathleen A. Trychta; Emily S. Wires; Raven M. Osborn; Justin R. Leach; Zoha F. Faraz; Reinis Svarcbahs; Christopher T. Ri...; Stephen Dewhurst; Brandon K. Harvey
• Keywords: Secretory protein; Proteostasis; Transport protein; COPI; COPII; Cellular compartment

Abstract SARS-CoV-2 E and 3a proteins are important for the assembly, budding, release of viral particles. These two transmembrane have been implicated in forming channels membrane that allow transport ions to favor replication. During an active infection, both generally localize endoplasmic reticulum (ER), ER-Golgi intermediate compartment (ERGIC), Golgi where assembly occurs. The ER critical proper packaging trafficking cellular along secretory pathways which determine a protein’s final destination inside or outside cell. virus primarily infects epithelial cells highly nature such as those lung gut. Here we quantified distribution human intestinal cell line. We used NaturePatternMatch demonstrate epitope-tagged expressed alone via transient transfection similar immunoreactivity pattern by wild-type infection. While localized with all selected markers varying degrees, protein displayed higher correlation coefficient Golgi, early/late endosome, lysosome, plasma when compared protein. This work is first provide quantification subcellular multiple components pathway serves basis develop models examining how alter proteostasis within these structures affect their function.