Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes

• DOI: 10.1007/s10753-017-0712-4
• Publication Date: 2017-12-14T00:39:48Z
• Authors: Xiaojian Huang; Qiyong Pan; Zekai Mao; Rui Zhang; Xiaohu Ma; Yang Xi; Hongbo You
• Keywords: Inflammation; 0301 basic medicine; 0303 health sciences; Coumaric Acids; Anti-Inflammatory Agents; Interleukin-18; Down-Regulation; Nitric Oxide; Dinoprostone; Rats; 3. Good health; 03 medical and health sciences; Chondrocytes; Osteoarthritis; Animals; Mitogen-Activated Protein Kinases; Cells, Cultured

Osteoarthritis (OA) is a degenerative joint disease frequently seen in the elderly population. Sinapic acid (SA), a commonly found phenolic acid, has been pharmacologically evaluated for its anti-inflammation effects in various studies. To explore its potential therapeutic role for OA, rat chondrocytes were treated with IL-1β (10 ng/ml) with different concentrations of SA in vitro. Our study revealed that SA could inhibit the IL-1β-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2). Consistent with these findings, the increased protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase (Cox)-2 could also be downregulated by SA. Moreover, SA could also suppress the IL-1β-induced expression of matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) in chondrocytes. Furthermore, our data found that SA could suppress the IL-1β-induced mitogen-activated protein kinase (MAPK) pathway activation. In general, this paper elucidates that sinapic acid inhibits the IL-1β-induced inflammation via MAPK pathways and may be a good agent for the treatment of OA.