Protective Effects of Sweroside on IL-1β-Induced Inflammation in Rat Articular Chondrocytes Through Suppression of NF-κB and mTORC1 Signaling Pathway

• DOI: 10.1007/s10753-018-0906-4
• Publication Date: 2018-10-12T04:25:28Z
• Authors: Rui Zhang; Chao-min Wang; Hua-ji Jiang; Xing-gui Tian; WenJun Li; Wei Liang; Jianhua Yang; Chunlan Zhong; Yuhui Chen; Tao Li
• Keywords: Cartilage, Articular; Inflammation; 0301 basic medicine; Plants, Medicinal; Interleukin-1beta; Iridoid Glucosides; Anti-Inflammatory Agents; NF-kappa B; Mechanistic Target of Rapamycin Complex 1; Nitric Oxide; Protective Agents; Dinoprostone; Rats; 3. Good health; 03 medical and health sciences; Chondrocytes; Animals; Signal Transduction

Sweroside (SW), as a bioactive herbal ingredient, has anti-inflammatory effects. Protective effects of SW on IL-1β-stimulated articular chondrocytes, however, has not been fully understood. This study was to explore the anti-inflammatory effects and further to investigate the possible mechanism underlying SW effect on IL-1β-stimulated rat articular chondrocytes. Rat articular chondrocytes were cultured with or without SW for 1 h, and then stimulated with IL-1β for 24 h. ELISA analysis was used to measure the production of NO and PGE2. Western blot was to detect the expression of iNOS and COX-2. Furthermore, the mRNA expression of MMP-1, MMP3, MMP13, and ADAMTS-5 were measured by q-PCR. These results demonstrated that SW significantly inhibited IL-1β-induced NO and PGE2 production, as well as MMP-1, MMP3, MMP13, and ADAMTS-5 mRNA expression. Moreover, SW also suppressed IL-1β-induced NF-κB activation and iκ-B degradation, S6K1 and S6 phosphorylation. In conclusion, these results strongly demonstrated that the anti-inflammatory activity of SW is in part mediated by suppressing NF-κB and mTORC1 signaling, which was expected to be a promising drug target of osteoarthritis therapy.