Puerarin Ameliorates Sarcopenia in Aged Mice via Modulation of Inflammation and Oxidative Stress: Insights from Proteomics and Transcriptomics

• DOI: 10.1007/s10753-025-02274-9
• Publication Date: 2025-03-24T11:44:21Z
• Authors: Shangjin Lin; Ying Cheng; Tao Cui; Fengjian Yang; Shengwu Yang; Yongqian Fan
• Keywords: Puerarin; Muscle Atrophy

Sarcopenia, a chronic degenerative condition associated with aging, is characterized by significant decline in muscle mass and strength. Puerarin, major active isoflavone extracted from Pueraria lobata, exhibits potent anti-inflammatory antioxidant properties. However, its therapeutic effects on sarcopenia remain unclear. Thus, the purpose of this study was to evaluate underlying molecular mechanisms puerarin ameliorating naturally aged mice. Twenty-month-old male C57BL/6 J mice were randomly divided into two groups based body weight: group (puerarin dissolved double-distilled water, 150 mg/kg/day) control (equal volume water). After an 8-week intervention, changes function between compared. Techniques such as HE staining, immunofluorescence ELISA, transmission electron microscopy, Western blot, qRT-PCR employed positive Furthermore, serum proteomics transcriptomics used analyze anti-muscle atrophy puerarin. The results demonstrated that significantly improved composition, enhanced function, exerted modulating inflammatory cytokines, reducing oxidative stress, inhibiting expression apoptosis proteins skeletal muscle. Additionally, integrated analyses suggested might be related TNF-α/NF-κB signaling pathway. These findings highlight puerarin's potential agent for sarcopenia, providing foundation further research clinical application.