Preparation and preliminary cytocompatibility of magnesium doped apatite cement with degradability for bone regeneration

• DOI: 10.1007/s10856-011-4228-4
• Publication Date: 2011-01-21T07:33:39Z
• Authors: Jingxiong Lu; Jie Wei; Yonggang Yan; Hong Li; Junfeng Jia; Shicheng Wei; Han Guo; Tiqiao Xiao; Changsheng Liu
• Keywords: Ions; Bone Regeneration; Time Factors; Compressive Strength; Bone Cements; Biocompatible Materials; Cell Differentiation; 02 engineering and technology; Alkaline Phosphatase; Bone and Bones; Phenotype; Models, Chemical; X-Ray Diffraction; Apatites; Humans; Magnesium; Hydroxyapatites; Stress, Mechanical; Powders; 0210 nano-technology; Cell Proliferation

In the present study, we fabricated magnesium doped apatite cement (md-AC) with rapid self-setting characteristic by adding the mixed powders of magnesium oxide and calcium dihydrogen phosphate (MO-CDP) into hydroxyapatite cement (HAC). The results revealed that the md-AC with 50 wt% MO-CDP could set within 6 min and the compression strength could reach 51 MPa after setting for 1 h, indicating that the md-AC had highly initial mechanical strength. The degradability of the md-AC in Tris-HCl solution increased with the increase of MO-CDP amount, and the weight loss ratio of md-AC with 50 wt% MO-CDP was 57.5 wt% after soaked for 12 weeks. Newly flake-like apatite could be deposited on the md-AC surfaces after soaked in simulated body fluid (SBF) for 7 days. Cell proliferation ratio of MG(63) cells on md-AC was obviously higher than that of HAC on days 4 and 7. The cells with normal phenotype spread well on the md-AC surfaces and attached intimately with the substrate, and alkaline phosphatase (ALP) activity of the cells on md-AC significantly improved compared with HAC on day 7. The results demonstrate that the md-AC has a good ability to support cell proliferation and differentiation, and indicate a good cytocompatibility.