Improved healing of critical-size femoral defect in osteoporosis rat models using 3D elastin/polycaprolactone/nHA scaffold in combination with mesenchymal stem cells

• DOI: 10.1007/s10856-021-06495-w
• Publication Date: 2021-03-08T11:02:49Z
• Authors: Fatemeh Hejazi; Vahid Ebrahimi; Mehrdad Asgary; Abbas Piryaei; Mohammad Javad Fr...; Ali Asghar Kermani; Fatemeh Zare; Mohammad-Amin Abd...
• Keywords: Polycaprolactone

Abstract Osteoporosis is a common bone disease that results in elevated risk of fracture, and delayed healing impaired regeneration are implicated by this disease. In study, Elastin/Polycaprolactone/nHA nanofibrous scaffold combination with mesenchymal stem cells were used to regenerate defects. Cytotoxicity, cytocompatibility cellular morphology evaluated vitro observations revealed an appropriate environment for attachment, growth, migration, proliferation provided scaffold. At 3 months following ovariectomy (OVX), the rats as animal models induced critical size defect femur evaluate therapeutic potential osteogenic differentiation marrow (BM-MSCs) seeded on dimension (3D) scaffolds. experimental 24 female Wistar equally divided into three groups: Control, (non-seeded BM-MSC), + cell (seeded BM-MSC) groups. 30 days after surgery, right was removed, underwent stereological analysis RNA extraction order examine expression Bmp-2 Vegf genes. The showed significant increase parameters groups compared control rats. present study suggests use 3D Elastin/Polycaprolactone (PCL)/Nano hydroxyapatite (nHA) MSCs may improve fracture accelerates at osteotomy site