Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro study

• DOI: 10.1007/s10856-022-06651-w
• Publication Date: 2022-02-21T18:09:51Z
• Authors: Shayanti Mukherjee; Smriti Sharma; Vivek Soni; Amruta Joshi; Amit Gaikwad; Jayesh Bellare; Jyoti Kode
• Keywords: Titanium; 0301 basic medicine; Chitosan; Osteoblasts; Surface Properties; Osteoblast; Silicates; Systematic in vitro approach; Calcium Compounds; Nanocomposites; 03 medical and health sciences; Coated Materials, Biocompatible; Biomimetic material; Osseointegration; Apatites; TA401-492; Medical technology; Dental/Orthopedic implant; R855-855.5; Biocompatibility Studies; Materials of engineering and construction. Mechanics of materials

AbstractBackgroundThere is a continuous research in the area of biomimetic coatings on the titanium (Ti) implant surfaces for improved survival and long-term successful outcomes in the field of dentistry and orthopedics. In-vitro approaches are ideal systems for studying cell-material interactions without complexity and interference observed in in-vivo models.PurposeThe present study was undertaken to evaluate the osteoblast characteristics and function on Ti substrates coated with the novel composite coating of ceramic apatite-wollastonite (AW) and polymer chitosan.Materials and methodsTi substrate coated with composite AW-Chitosan was synthesized, using electrophoretic deposition. MG-63 cells were seeded onto the coated substrates and cellular morphology and growth was assessed using Scanning Electron Microscopy (SEM) and Laser Scanning Microscopy (LSM). Osteocalcin expression of the seeded cells was assessed by FITC tagging and LSM analysis. Alizarin Red S staining and Confocal LSM (CSLM) analysis was used to study the in-vitro mineralization on the titanium samples.ResultsThe AW-Chitosan coating on Ti samples by electrophoretic deposition exerted significant positive influence on cell proliferation, growth and mineralization as compared to uncoated titanium samples. Scanning electron microscopy and laser confocal microscopy experiments revealed that the coating was non-toxic to cells, enhanced adhesion and proliferation of MG-63 cells. Increased functional activity was observed by increased production of bone-specific protein osteocalcin and mineralized calcium through day 7 and 14.ConclusionsThe present study underscores that optimal inorganic-organic phase nanocomposite crack-free coating created on Ti by simple, cost-effective electrophoretic deposition technique may have osteoconductive potential and may have wide application in the field of implantology.