Aspirin/PLGA coated 3D-printed Ti-6Al-4V alloy modulate macrophage polarization to enhance osteoblast differentiation and osseointegration

• DOI: 10.1007/s10856-022-06697-w
• Publication Date: 2022-10-08T18:02:30Z
• Authors: Yapeng You; Wanmeng Wang; Ying Li; Yunjia Song; Jian Jiao; Yao Wang; Bo Chen; Jialin Liu; Hui Qi; Yu Liang
• Keywords: PLGA; Bioceramic

Although titanium (Ti) and Ti-based alloy have been widely used as dental orthopedic implant materials, its bioinertness hindered the rapid osseointegration. Therefore, it is recommended to acquire ideal topographic chemical characteristics through surface modification methods. 3D printing a delicate manufacture technique which possesses superior controllability reproducibility. While aspirin serve well-established non-steroidal anti-inflammatory agent. Recently, importance of immune system in regulating bone dynamics has attracted increasing attention. We herein superimposed aspirin/poly (lactic-co-glycolic acid) (ASP/PLGA) coating on 3D-printed Ti-6Al-4V with uniform micro-structure establish Ti64-M-ASP/PLGA substrate. Scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) contact angle test confirmed successful fabrication substrate, increased wettability sustained release pattern ASP. Compared Ti64 base material, substrate showed enhanced M2 depressed M1 genes proteins expressions macrophages. The novel also displayed osteoblast proliferation, adhesion, extracellular mineralization ability osteogenic gene when cultured macrophage conditioned medium vitro. Furthermore, rat femora implantation model was for vivo evaluation. After 4 weeks implantation, push out test, micro-computed tomography (micro-CT) histological analyses all osseointegration capabilities than other groups. Our study revealed synergistic role played by micro topography immunoregulatory drug promoting osteogenesis vitro accelerating vivo, thus providing promising method better modifying surface. Graphical abstract.