Mitochondrial dysfunction and effect of antiglycolytic bromopyruvic acid in GL15 glioblastoma cells
Membrane Potential, Mitochondrial
0301 basic medicine
Cytochromes c
Apoptosis
Free Radical Scavengers
Acetylcysteine
Mitochondria
03 medical and health sciences
Adenosine Triphosphate
GL15 cells, Cytochrome c, ROS, Mitochondrial membrane potential, Bromopyruvate, Respiratory chain inhibitors
Cell Line, Tumor
Mitochondrial Membranes
Proteolysis
Humans
Enzyme Inhibitors
Glioblastoma
Pyruvates
Reactive Oxygen Species
Glycolysis
Oxidation-Reduction
DOI:
10.1007/s10863-011-9375-2
Publication Date:
2011-07-20T10:39:43Z
AUTHORS (9)
ABSTRACT
Most cancer cells, including GL15 glioblastoma cells, rely on glycolysis for energy supply. The effect of antiglycolytic bromopyruvate on respiratory parameters and viability of GL15 cells was investigated. Bromopyruvate caused Δψ(m) and MTT collapse, ATP decrease, and cell viability loss without involving apoptotic or necrotic pathways. The autophagy marker LC3-II was increased. Δψ(m) decrease was accompanied by reactive oxygen species (ROS) increase and cytochrome c (cyt c) disappearance, suggesting a link between free radical generation and intramitochondrial cyt c degradation. Indeed, the free radical inducer menadione caused a decrease in cyt c that was reversed by N-acetylcysteine. Cyt c is tightly bound to the inner mitochondrial membrane in GL15 cells, which may confer protein peroxidase activity, resulting in auto-oxidation and protein targeting to degradation in the presence of ROS. This process is directed towards impairment of the apoptotic cyt c cascade, although cells are committed to die.
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