Catalytic activity and stability of laccase entrapped in sol–gel silica with additives

• DOI: 10.1007/s10971-011-2596-3
• Publication Date: 2011-10-07T15:26:24Z
• Authors: Nur Atikah Mohidem; Hanapi Bin Mat
• Keywords: QD Chemistry; 16. Peace & justice; 01 natural sciences; 620; 0104 chemical sciences

This study investigated the effects of different additives and precursors on the catalytic activity of laccase entrapped in sol–gel silica. It was found that the laccase catalytic activity and stability of sol–gel laccase could be enhanced if the entrapment was performed in the presence of additives such as PVA, PEG and APTS. The use of TEOS as a precursor showed slightly higher laccase catalytic activity compared to TMOS. The PVA as an additive showed a better catalytic activity enhancement compared to the PEG and APTMS with the optimum PVA concentration of 0.03 mg/mL. The optimal temperatures of sol–gel laccase without and with additives were found to be at 40 and 27°C, respectively. After 70 days of storage at 27°C, the catalytic activity of the immobilized sol–gel laccase with additives maintained its catalytic activity compared to only 30% of its original catalytic activity for the sol–gel laccase without additives.