Novel human BTB/POZ domain-containing zinc finger protein ZBTB1 inhibits transcriptional activities of CRE
Cell Nucleus
0301 basic medicine
0303 health sciences
Base Sequence
Sequence Homology, Amino Acid
Transcription, Genetic
Molecular Sequence Data
Zinc Fingers
Sequence Analysis, DNA
Response Elements
Protein Structure, Tertiary
Repressor Proteins
03 medical and health sciences
Gene Expression Regulation
COS Cells
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Cyclic AMP Response Element-Binding Protein
HeLa Cells
Signal Transduction
DOI:
10.1007/s11010-011-0911-5
Publication Date:
2011-06-25T15:21:09Z
AUTHORS (9)
ABSTRACT
BTB/POZ protein family plays a key role in many biological processes by regulating the transcriptional activities of some downstream genes. Here, we characterized the member of C(2)H(2) type zinc finger gene, Zinc finger and BTB domain containing 1 (ZBTB1). The complete sequence of ZBTB1 cDNA contains a 2142 bp open reading frame (ORF) and encodes a 713 amino acid protein with an N-terminal BTB/POZ domain that is similar to the same domain of other known transcription regulators and eight classical zinc finger C(2)H(2) motifs in the C-terminus. Subcellular localization analysis demonstrated that ZBTB1 was localized to the nucleus, forming dot-like structures. Transcriptional activity assays showed that ZBTB1 was a transcription repressor and overexpression of ZBTB1 in the COS7 cells reduced the transcriptional activities of cAMP response element (CRE). Further studies showed that the BTB domain and ZNF motifs of ZBTB1 may both be involved in this suppression. These results suggest that ZBTB1 protein may act as a transcription repressor in the activation of CREB and cAMP-mediated signal transduction pathways to mediate cellular functions.
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