Dynamics of telopodes (telocyte prolongations) in cell culture depends on extracellular matrix protein

• DOI: 10.1007/s11010-014-2215-z
• Publication Date: 2014-09-20T08:39:16Z
• Authors: Cristina Mariana ...; T. M. Regalia; Mihaela Gherghiceanu; R. Huica; Mihaela Surcel; C. Ursaciuc; M. Leabu; L. M. Popescu
• Keywords: Matrix (chemical analysis); Dynamics

Telocytes (TC) are cells with telopodes (Tp), very long prolongations (up to 100 μm) an uneven caliber ( www.telocytes.com ). Factors determining the dynamics of cellular still unknown, although previous studies showed telopode motility in TC cultures. We comparatively investigated, by time-lapse videomicroscopy, Tp mouse heart seeded on collagen, fibronectin, and laminin. Under our experimental conditions, fibroblasts (cell line L929) behaved differently terms adherence, spreading, prolongation extension. Fibroblasts lower spreading matrix proteins used. The time needed for was 2-4 h TC, versus 8-10 fibroblasts. values final cell surface area after were between 200 400 μm(2) 800-2,000 TC. a more than three times higher ability spread tested proteins. An extremely low capacity extend lengths shorter bodies noted fibroblasts, while extended longer body length, moniliform appearance. stronger adherence lowest Collagen determined intermediate but highest extensions. In conclusion, behave extension when various culture.