Diversity of actin architecture in human osteoclasts: network of curved and branched actin supporting cell shape and intercellular micrometer-level tubes

• DOI: 10.1007/s11010-017-3004-2
• Publication Date: 2017-03-14T07:09:34Z
• Authors: Paula Pennanen; Maria Helena Alanne; Elnaz Fazeli; Takahiro Deguchi; Tuomas Näreoja; Sirkku Peltonen; Juha Peltonen
• Keywords: Podosome; STED microscopy; Cortactin; Cofilin; Multinucleate; Actin remodeling

Osteoclasts are multinucleated bone-resorbing cells with a dynamic actin cytoskeleton. derived from circulating mononuclear precursors. Confocal and stimulated emission depletion (STED) super-resolution microscopy was used to investigate peripheral blood-derived human osteoclasts cultured on glass surfaces. STED confocal demonstrated that the curved branched, for instance, in vicinity of membrane ruffles. The overall architecture network extended podosomes top cell. other novel finding micrometer-level tube containing bridged well above level culture glass. filaments tubes originated often surrounding group nuclei. Furthermore, nuclei were occasionally located inside tubes. Our findings accumulation c-Src, cortactin, cofilin, around suggesting their role nuclear processes such as locomotion ARP2/3 labeling abundant at substratum branched network, where it localized branching points. We speculate actin-containing may provide means transportation nuclei, e.g., during fusion osteoclasts. These can pave way future studies aiming elucidation differentiation