A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers

• DOI: 10.1007/s11032-007-9083-0
• Publication Date: 2007-02-22T09:54:25Z
• Authors: Juliana Stein; Silvina C. Pessino; Eric J. Martínez; María Pía Rodriguez; Lorena A. Siena; Camilo L. Quarin; Juan Pablo Amelio...
• Keywords: Paspalum Notatum; 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; Mapping in autopolyploids; https://purl.org/becyt/ford/1.6; Paspalum notatum; Apospory; Mapping in Autopolyploids; Single Dose Markers; https://purl.org/becyt/ford/1; Single dose markers

Paspalum notatum Flugge is a warm- season forage grass with mainly diploid (2n = 20) and autotetraploid (2n = 40) representa- tives. Diploid races reproduce sexually and require crosspollination due to a self-incom- patible mating system, while autotetraploids reproduce by aposporous apomixis. The objec- tives of this work were to develop a genetic linkage map of Paspalum notatum Flugge at the tetraploid level, identify the linkage/s group/s associated with apomixis and carry out a general characterization of its mode of inheritance. A pseudo test-cross F1 family of 113 individuals segregating for the mode of reproduction was obtained by crossing a syn- thetic completely sexual tetraploid plant (Q4188) as female parent with a natural aposporous individual (Q4117) as pollen donor. Map construction was based on single-dose markers (SDAFs) segregating from both par- ents. Two linkage maps (female and male) were constructed. Within each map, homolo- gous groups were assembled by detecting repulsion-phase linked SDAFs. Putative Q4188 and Q4117 homolog groups were identified by mapping shared single dose mark- ers (BSDF). The Q4188 map consisted of 263 markers distributed on 26 co-segregation groups over a total genetic distance of 1.590.6 cM, while the Q4117 map contained 216 loci dispersed on 39 co-segregation groups along 2.265.7 cM, giving an estimated genome cov- erage of 88% and 83%, respectively. Seven and 12 putative homologous chromosomes were detected within Q4188 and Q4117 maps, respectively. Afterward, ten female and male homologous chromosomes were identified by mapping BSDFs. In the Q4117 map, a single linkage group was associated with apospory. It was characterized by restriction in recombina- tion and preferential chromosome pairing. A BPSD marker mapping within this group allowed the detection of the female homolog and the putative four male groups of the set carrying apospory.