Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response

• DOI: 10.1007/s11032-014-0095-2
• Publication Date: 2014-04-28T15:23:54Z
• Authors: Carla C. Silva; Camila C. Mantello; Tatiana Campos; Livia M. Souza; Paulo S. Gonçalves; Anete P. Souza
• Keywords: Hevea

Hevea brasiliensis is a native species of the Amazon Basin South America and primary source natural rubber worldwide. Due to occurrence American Leaf Blight disease in this area, plantations have been extended suboptimal regions. Rubber tree breeding time-consuming expensive, but molecular markers can serve as tool for early evaluation, thus reducing time costs. In work, we constructed six different cDNA libraries with aim developing gene-targeted tree. A total 8,263 reads were assembled, generating 5,025 unigenes that analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, two sequences highly up-regulated by cold stress. These scanned microsatellite (SSR) regions single nucleotide polymorphisms (SNPs). total, 169 novel EST-SSR developed; 138 loci polymorphic tree, 98 % presented transferability other species. Locus duplication was observed H. Additionally, 43 SNP 13 showed similarity proteins involved stress response, latex biosynthesis developmental processes characterized. are rich SSR enable identification transcripts. The developed here will be valuable resource linkage mapping, QTL studies also used evaluate genetic variability species, which assets breeding.