Expression of EGFP-spider dragline silk fusion protein in BmN cells and larvae of silkworm showed the solubility is primary limit for dragline proteins yield

• DOI: 10.1007/s11033-007-9090-6
• Publication Date: 2007-05-25T09:02:14Z
• Authors: Yuansong Zhang; Junhua Hu; Yungen Miao; Aichun Zhao; Tianfu Zhao; Dayang Wu; Liefeng Liang; Ayumi Miikura; Kunihiro Shiomi; Zenta Kajiura; Masao Nakagaki
• Keywords: 0303 health sciences; DNA, Complementary; Base Sequence; Recombinant Fusion Proteins; Molecular Sequence Data; Gene Expression; Spiders; Bombyx; Cell Line; 03 medical and health sciences; Solubility; Genes, Reporter; Larva; Animals; Amino Acid Sequence; Fibroins

Spider dragline silk is a unique fibrous protein with combination of tensile strength and elasticity, but the isolation of large amount of silk from spiders is not feasible. In this paper, we used a newly established Bac-to-Bac/BmNPV Baculovirus expression system to express the recombinant spider (Nephila clavata) dragline silk protein (MaSp1) fused EGFP in BmN cells and larvae of silkworm. A 70 kDa fusion protein was visualized after rBacmid/BmNPV/drag infection by SDS-PAGE and immunoblotting analysis. Fusion protein expressed in the BmN cells probably occupied five percent of the cell total protein; In a silkworm larva, approximately 6 mg fusion proteins were expressed. Solubility analysis of the expressed spider dragline silk protein indicated that 60% fusion protein is insoluble. EGFP fluorescence showed that fusion protein is tend to form aggregate by self assemblage. The results indicated the solubility is the primary limit for spider dragline proteins yield. It also suggested that directly produce fibrous spider silk in the secreting-silk organs of the transgenic silkworm larvae might be a better method.