Association of Cytotoxic T Lymphocyte-associated antigen-4 gene haplotype with the susceptibility to gastric cancer

• DOI: 10.1007/s11033-009-9705-1
• Publication Date: 2009-08-17T02:47:48Z
• Authors: Ruiping Hou; Bangwei Cao; Zhongdong Chen; Yong Li; Tao Ning; Chunhui Li; Changqing Xu; Ziping Chen
• Keywords: Male; 0301 basic medicine; 0303 health sciences; Middle Aged; Polymorphism, Single Nucleotide; 3. Good health; 03 medical and health sciences; Haplotypes; Antigens, CD; Stomach Neoplasms; Case-Control Studies; Humans; CTLA-4 Antigen; Female; Genetic Predisposition to Disease

Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) was widely accepted as a pivotal molecule in downregulating T-cell mediated immune responses. In this study we investigated the polymorphisms which would impact the CTLA-4 gene expression and function to assess the association with the risk of gastric cancer. 205 gastric cancer patients and 262 healthy controls were included in the case-control study. PCR and restriction fragment length polymorphism (RFLP) methods were performed to identify the +49A/G and promoter -1661A/G polymorphisms. The promoter -1772T/C polymorphism was detected by PCR amplification refractory mutation system (ARMS) technique. A significant difference was observed between case and control groups. The frequency of +49A/G polymorphism AG and -1661A/G polymorphism GG genotype were significantly higher in patients than in controls (OR = 2.15, OR = 1.88, respectively). No significant difference was found in the allelic frequency of -1772T/C polymorphism between cases and controls (P = 0.478). By the haplotype analysis, logistic regression showed the frequency of haplotype A (GAT) and D (AGT) in the case group revealed significant difference compared with in control group (OR = 2.00, P < 0.001; OR = 1.62, P = 0.043, respectively). Our findings implied the genetic variations within CTLA-4 gene would be a critical risk factor to the susceptibility of gastric cancer.