Molecular cloning and expression analysis of mannose receptor in blunt snout bream (Megalobrama amblycephala)

• DOI: 10.1007/s11033-014-3331-2
• Publication Date: 2014-04-16T10:22:41Z
• Authors: Xiaoling Liu; Xiaocheng Tang; Li Wang; Jie Li; Hong Wang; Shun Wei; Rui Fang; Wei Ji; Gailing Yuan; Nan Chen; Zemao Gu; Xueqin Liu; Weimin Wang; Muhammad Asim; Yang Zhou; Li Lin
• Keywords: 0301 basic medicine; Base Sequence; Molecular Sequence Data; Cyprinidae; Gene Expression; Receptors, Cell Surface; Recombinant Proteins; Aeromonas hydrophila; Protein Structure, Tertiary; Fish Diseases; Mice; 03 medical and health sciences; Mannose-Binding Lectins; Animals; Humans; Lectins, C-Type; Amino Acid Sequence; Cloning, Molecular; Chickens; Sequence Alignment; Mannose Receptor

Mannose receptor (MR) plays a significant role in innate immune responses to pathogens in vertebrates. Here we characterized the first teleost MR from Megalobrama amblycephala, named maMR and its expression patterns were investigated. The full-length maMR consists of 5,295 bp encoding a putative protein of 1,433 amino acids. The predicted amino acid sequences showed that maMR contained a signal peptide, a cysteine-rich domain, a single fibronectin type II domain, eight tandemly arranged C-type lectin-like domains, a transmembrane domain and a C-terminal cytoplasmic domain. Phylogenetic analysis revealed the highest similarity of maMR with Danio rerio MR predicted by computational analysis. The maMR-mRNAs were ubiquitously transcribed in different tissues, However the highest transcripts were observed in head kidney. Transcripts of maMR significantly increased at the late stages of embryo and continued to be at the high levels after hatching. The maMR transcripts were significantly increased in M. amblycephala after stimulation with killed Aeromonas hydrophila.