Overexpression of Zostera japonica heat shock protein gene ZjHsp70 enhances the thermotolerance of transgenic Arabidopsis
Thermotolerance
0301 basic medicine
Arabidopsis Proteins
Zosteraceae
Arabidopsis
Plants, Genetically Modified
Antioxidants
03 medical and health sciences
Gene Expression Regulation, Plant
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
Heat-Shock Response
Plant Proteins
DOI:
10.1007/s11033-022-07411-3
Publication Date:
2022-04-12T16:55:31Z
AUTHORS (2)
ABSTRACT
Heat shock protein 70s (Hsp70s) are major members of the heat shock protein family and play a variety of roles to protect plants against stress. Plant Hsp70s are a conserved and widely expressed family of heat shock proteins. They have two main functional regions: N-terminal nucleic acid binding region and C-terminal substrate binding region.In this study, we cloned the Hsp70 gene of Zostera japonica (ZjHsp70) based on the sequence obtained by transcriptome sequencing. The transcriptional levels of ZjHsp70 increased significantly at 1 h after heat treatment. ZjHsp70 was located in the cytoplasm and nucleus. The overexpression of ZjHsp70 in Arabidopsis resulted in increased heat tolerance, lower contents of malondialdehyde and higher antioxidant enzyme activity than in the wild type. ZjHsp70 may achieve this goal by maintaining highly active antioxidant enzymes.We show that ZjHsp70 can improve plant heat tolerance by maintaining high antioxidant enzyme activity under high temperature stress. This study provided a basis to study the role of ZjHsp70 in thermotolerance in more detail.
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