Transcriptomic analysis of bakanae disease resistant and susceptible rice genotypes in response to infection by Fusarium fujikuroi

• DOI: 10.1007/s11033-022-07877-1
• Publication Date: 2022-10-21T19:02:42Z
• Authors: Bishnu Maya Bashyal; Kirti Rawat; Pooja Parmar; Ashish Kumar Gupta; Sangeeta Gupta; S Gopala Krishnan; Ravish Choudhary; Sezai Ercisli; Antonija Kovacevic; Rashmi Aggarwal
• Keywords: 0301 basic medicine; Genotype; Plant disease resistance; Plant Science; Gene; Microbiology; Biochemistry; Agricultural and Biological Sciences; 03 medical and health sciences; Fusarium; Biochemistry, Genetics and Molecular Biology; Genetics; Mycotoxins and Their Impact; Biology; Plant Diseases; 2. Zero hunger; 0303 health sciences; Life Sciences; Oryza; Cell Biology; Fusarium oxysporum; FOS: Biological sciences; Transcriptome; Genetics and Epidemiology of Plant Pathogens; Transcriptome Analysis; Hypothetical protein; Diversity and Evolution of Fungal Pathogens

Abstract Background Fusarium fujikuroi causing bakanae is one of the most significant pathogen of rice and much responsible for yield losses thereby emerging as a major risk to food security.Methods In the present study transcriptomic analysis was conducted between two contrasting resistant (C101A51) and susceptible (Rasi) genotypes of rice with the combinations of C101A51 control (CC) vs C101A51 inoculated (CI); Rasi control (RC) vs Rasi inoculated (RI) and C101A51 inoculated (CI) vs Rasi inoculated (RI). Results In CC vs CI commonly expressed genes were 12,764. Out of them 567 (4%) were significantly upregulated and 1399 (9%) genes were down regulated. For the RC vs RI 14, 333 (79%) genes were commonly expressed. For CI vs RI 13,662 (72%) genes commonly expressed. Cysteine proteinase inhibitor 10, disease resistance protein TAO1-like, oleosin 16 kDa-like, pathogenesis-related protein (PR1), (PR4), BTB/POZ and MATH domain-containing protein 5-like, alpha-amylase isozyme 3D-like (LOC4345814), were upregulated in resistant genotype C101A51. Whereas, GDSL esterase/lipase At5g33370, serine glyoxylate aminotransferase, CASP-like protein 2C1, WAT1-related protein At4g08290, Cytoplasmic linker associated proteins, xyloglucan endotransglucosylase/hydrolase protein and β-D xylosidase 7 were upregulated in susceptible genotype Rasi. Gene ontology analysis showed functions related to defence response (GO:0006952), regulation of plant hypersensitive type response (GO:0010363), Potassium ion transmembrane activity (GO:0015079), chloroplast (GO:0009507), response to wounding (GO:0009611), xylan biosynthetic process (GO:0045492) were upregulated in resistant genotype C101A51 under inoculated conditions.Conclusions Real time PCR based validation of the selected DEGs showed that the qRT-PCR was consistent with the RNA-Seq results. This is the first transcriptomic study against bakanae disease of rice in Indian genotypes and will be helpful for the development of bakanae management strategies.