Dietary phytochemical indole-3-carbinol regulates metabolic reprogramming in mouse prostate tissue

• DOI: 10.1007/s11095-025-03820-8
• Publication Date: 2025-02-04T21:14:28Z
• Authors: Rebecca Mary Peter; Md. Shahid Sarwar; Lujing Wang; Pochung Chou; Chao Wang; Yujue Wang; Xiaoyang Su; Ah-Ng Kong
• Keywords: Phytochemical; Reprogramming

Indole-3-carbinol (I3C) is shown to possess multiple pharmacological activities such as anti-inflammatory, antimicrobial, antioxidant, antiviral, and anti-cancer activities. It widely accepted modulator of signaling pathways particularly those related cell cycle, growth division, angiogenesis, apoptosis immunity. We explored the metabolic reprogramming based on treatment with I3C in mice prostate tissue. In this study we utilized Pten knockout (KO)-induced tumorigenesis mouse model examine mechanism action via rewiring. Phosphatase tensin homolog deleted chromosome 10 (Pten), a tumor suppressor gene frequently found be mutated or cancer. Untargeted metabolomics was performed using liquid-chromatography mass-spectrometry (LC-MS) platform investigate Pten-dependent Pten-independent targets I3C. The most impacted by included pyrimidine metabolism, arginine proline porphyrin citrate cycle lipoic acid metabolism. These taken together help understanding overall health beneficial effects