Von Willebrand factor with increased binding capacity is associated with reduced platelet aggregation but enhanced agglutination in COVID-19 patients: another COVID-19 paradox?

• DOI: 10.1007/s11239-020-02339-6
• Publication Date: 2021-01-02T14:02:38Z
• Authors: Franco Ruberto; Antonio Chistolini; Mariaignazia Curreli; Giacomo Frati; Antonino G. M. Ma...; Giuseppe Biondi-Z...; Massimo Mancone; Sebastiano Sciarr...; Fabio Miraldi; Francesco Alessandri; Giancarlo Ceccarelli; Francesco Barone; Cristina Santoro; Domenico Alvaro; Francesco Pugliese; Fabio M. Pulcinelli; ; Alida Albante; Francesco Alessandri; Domenico Alvaro; Guido Antonelli; Fabio Araimo-Mors...; Daniela Auricchio; Francesco Barone; Federico Bilotta; Giuseppe Biondi-Z...; Katia Bruno; Alessandro Cappan...; Vincenzo Cardinale; Giancarlo Ceccarelli; Paola Celli; Antonio Chistolini; Stella Consolo; Claudia Croce; Beatrice Crocitti; Mariaignazia Curreli; Gabriella D’Ettorre; Lauri De Daniela; Francesco De Lazzaro; Francesco Fedele; Giacomo Frati; Gioacchino Galardo; Lorena Giannetti; Stefano Ianni; Carmela Imperiale; Viviana Maestrini; Eugenia Magnanimi; Federica Maldarelli; Massimo Mancone; Sabina Martelli; Antonino G. M. Ma...; Claudio Mastroianni; Teresa Messina; Fabio Miraldi; Elisa Pattelli; Filippo Pecorari; Serena Perrella; Mario Piazzolla; Monica Portieri; Francesco Pugliese; Fabio M. Pulcinelli; Fabiola Ratini; Claudia Ricci; Franco Ruberto; Pietro Santopietro; Guglielmo Tellan; Luca Titi; Paolo Tordiglione; Antonella Tosi; Fausto Trigilia; Cristina Santoro; Sebastiano Sciarr...
• Keywords: Ristocetin; Agglutination (biology); Platelet-poor plasma; von Willebrand Disease

Patients with Coronavirus-associated disease-2019 (COVID-19) display alterations of the hemostatic system and presence a prothrombotic status frequently leading to vascular complications. However, impact COVID-19 on platelet activity, aggregation agglutination still needs be clarified. We measured total levels von Willebrand factor (vWF) vWF binding glycoprotein (Gp) complex (GPIb-IX-V), in cohort patients admitted intensive care unit our Institution. Moreover, we evaluated response agonists (ADP, collagen, arachidonic acid) ristocetin. found that antigen active form platelets (vWF:RCo), were markedly increased these patients. These results associated higher rates induced by ristocetin, thereby indirectly indicating an capability bind platelets. Conversely, both ADP collagen was lower compared healthy volunteers. This study shows is vWF-induced but reduced responsivity stimuli. Our findings have translational relevance since adhesion may represent marker predict possible complications better delineate therapeutic strategies