Over-expression of OSRIP18 increases drought and salt tolerance in transgenic rice plants

0301 basic medicine 2. Zero hunger Drought stress Ectopic over-expression Ribosome-inactivating protein Ribosome Inactivating Proteins Oryza Salt Tolerance 15. Life on land Plants, Genetically Modified 630 6. Clean water Droughts 03 medical and health sciences Gene Expression Regulation, Plant Stress, Physiological High salinity stress Rice
DOI: 10.1007/s11248-011-9568-9 Publication Date: 2011-10-25T18:40:21Z
ABSTRACT
Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for such improvement. Here, we have investigated the biological functions of an Oryza sativa Ribosome-inactivating protein gene 18 (OSRIP18) by ectopically expressing this gene under the control of CaMV 35S promoter in the rice genome. We have generated 11 independent transgenic rice plants and all of them showed significantly increased tolerance to drought and high salinity stresses. Global gene expression changes by Microarray analysis showed that more than 100 probe sets were detected with up-regulated expression abundance while signals from only three probe sets were down-regulated after over-expression of OSRIP18. Most of them were not regulated by drought or high salinity stresses. Our data suggested that the increased tolerance to these abiotic stresses in transgenic plants might be due to up-regulation of some stress-dependent/independent genes and OSRIP18 may be potentially useful in further improving plant tolerance to various abiotic stresses by over-expression.
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