Isolation of Histophilus somni from the nasal exudates of a clinically healthy adult goat

• DOI: 10.1007/s11250-011-9792-6
• Publication Date: 2011-02-16T17:20:21Z
• Authors: Nelson Pérez-Romero; Francisco Aguilar...; Beatriz Arellano-...; Efrén Díaz-Aparicio; Rigoberto Hernánd...
• Keywords: DNA, Bacterial; 0301 basic medicine; Goats; Molecular Sequence Data; Polymerase Chain Reaction; 3. Good health; 03 medical and health sciences; RNA, Ribosomal, 16S; Haemophilus somnus; Animals; Nasal Cavity; Mexico

The nasal exudate from 42 goats of the Mixteca Region in the state of Puebla, Mexico, was evaluated. A strain was isolated after 4 days of incubation. This strain was identified according to its phenotypic characteristics and by means of a species-specific polymerase chain reaction (PCR), as well as by sequencing of the amplified product.The species-specific PCR amplified a 407-bp fragment of 16S RNAr subunit, and the product sequencing revealed 100% homology with Histophilus somni 129PT. The nucleotide sequence was deposited in the GenBank under accession number HM032735.This is the first worldwide isolation of H. somni from nasal exudates of a clinically healthy goat.