In vitro inhibitory effect of trichostatin A on canine grade 3 mast cell tumor

• DOI: 10.1007/s11259-011-9474-x
• Publication Date: 2011-04-06T19:10:39Z
• Authors: Marcia Kazumi Nag...; Daniel S. Sanches; Katia C. Pinello; Luciana Neves Torres; Gregory Mennecier; Andréia O. Latorre; Heidge Fukumasu; Maria Lucia Zaida...
• Keywords: Trichostatin A; Mast (botany)

Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction of G0/G1 and G2/M -phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent for the treatment of canine MCT.