Feasibility of 16S rRNA sequencing for cerebrospinal fluid microbiome analysis in cattle with neurological disorders: a pilot study

• DOI: 10.1007/s11259-022-09949-w
• Publication Date: 2022-06-27T05:02:32Z
• Authors: Sara Ferrini; Elena Grego; Ugo Ala; Giulia Cagnotti; Flaminia Valentini; Giorgia Di Muro; Barbara Iulini; Maria Cristina St...; Claudio Bellino; Antonio D’Angelo
• Keywords: Microbiological culture; Etiology

Bacterial infection of the central nervous system (CNS) in cattle requires prompt and adequate antimicrobial treatment. The current gold standard for antemortem etiological diagnosis is cerebrospinal fluid (CSF) culture, which often yields false negative results. CSF has long been considered a sterile district healthy patients, but this notion recently challenged. For pilot study, we used 16S rRNA gene sequencing to investigate microbial composition presenting with CNS disorders compare it between subjects infections other nature. study sample was 10 animals: 4 infectious-inflammatory diseases 6 disorders, based on definitive diagnosis. Since initial round PCR did not yield sufficient genetic material any samples, protocol modified increase its sensitivity. identified animals 2 groups were formed: an infectious inflammatory (n = 3) noninfectious group 3). most frequently expressed bacterial families Pseudomonadaceae (44.61%), Moraxellaceae (19.54%), Mycobacteriaceae (11.80%); genera Pseudomonas (45.42%), Acinetobacter (19.91%), Mycobacterium (12.01%). There no detectable differences samples from two groups. Sequencing DNA present possible only after increasing results showed presence community neurological disorders. Further studies, environment are included as controls, needed.