Using reduced representation libraries sequencing methods to identify cpDNA polymorphisms in European beech (Fagus sylvatica L)

• DOI: 10.1007/s11295-018-1313-6
• Publication Date: 2019-01-04T09:54:55Z
• Authors: Joanna Meger; Bartosz Ulaszewski; Giovanni G. Vendr...; Jaroslaw Burczyk
• Keywords: Population Genetics

Reduced representation genomic libraries (RRLs) are increasingly used to answer diverse questions in evolutionary biology, which remained unresolved otherwise. In the case of European beech (Fagus sylvatica L.), former applications nuclear and chloroplast genetic markers indicated that most populations Central, Eastern, Northern Europe have a very homogeneous structure. Thus, determining differentiation among F. at regional scale Central has challenge for biologists, mainly due lack variable markers. this study, we RAD-seq GBS approaches identify novel cpDNA polymorphisms could be helpful population structure geographical scale. Our datasets allowed us indicate finer stronger patterns spatial than previous studies. It is expected cpDNA-based phylogenetic using whole-genome sequencing able provide even more detailed picture species phylogeography ever before.