Metabonomics of human fecal extracts characterize ulcerative colitis, Crohn’s disease and healthy individuals

• DOI: 10.1007/s11306-014-0677-3
• Publication Date: 2014-05-31T11:57:08Z
• Authors: Jacob Tveiten Bje...; Yulan Wang; Fuhua Hao; Mehmet Coskun; Christian Ludwig; Ulrich Günther; Ole Haagen Nielsen
• Keywords: Metabolome; Dysbiosis

This study employs spectroscopy-based metabolic profiling of fecal extracts from healthy subjects and patients with active or inactive ulcerative colitis (UC) Crohn's disease (CD) to substantiate the potential use spectroscopy as a non-invasive diagnostic tool characterize metabolome in inflammatory bowel (IBD). Stool samples 113 individuals (UC 48, CD 44, controls 21) were analyzed by 1H nuclear magnetic resonance (NMR) (Bruker 600 MHz, Bruker BioSpin, Rheinstetten, Germany). Data principal component analysis orthogonal-projection latent structure-discriminant using SIMCA-P + 12 MATLAB. Significant differences found profiles making it possible differentiate between IBD UC CD. The metabolites holding differential power primarily belonged range amino acids, microbiota-related short chain fatty lactate suggestive an inflammation-driven malabsorption dysbiosis normal bacterial ecology. However, removal intestinal surgery anti-TNF-α antibody treatment eliminated discriminative regarding versus consequently demonstrates that NMR is able changes related dysbiosis. Intestinal medication are be accounted for future studies, seems factors importance process.