A rapid method for profiling of volatile and semi-volatile phytohormones using methyl chloroformate derivatisation and GC–MS

• DOI: 10.1007/s11306-015-0837-0
• Publication Date: 2015-09-08T02:41:41Z
• Authors: Catherine Rawlinson; Lars G. Kamphuis; Joel P. A. Gummer; Karam B. Singh; Robert D. Trengove
• Keywords: Profiling (computer programming); Chloroformate

Phytohormones are central components of complex signalling networks in plants. The interplay between these metabolites, which include abscisic acid (ABA), auxin (IAA), ethylene, jasmonic (JA) and salicylic (SA), regulate plant growth development modulate responses to biotic abiotic stress. Few methods phytohormone profiling can adequately quantify a large range hormones simultaneously without the requirement for laborious or highly specialised extraction protocols. Here we describe validation protocol, based on methyl-chloroformate derivatisation metabolites analysis by gas chromatography/mass spectrometry (GC-MS). We 11 either phytohormones intermediates metabolism; ABA, azelaic acid, IAA, JA SA, precursors 1-aminocyclopropane 1-carboxylic benzoic cinnamic 13-epi-12-oxophytodienoic (13-epi-OPDA), linoleic linolenic validate isolation from foliar tissue model legume Medicago truncatula. preparation is insensitive presence water, facilitating measurement volatile metabolites. Quantitation was linear over four orders magnitude, limits detection two 10 ng/mL all measured using single quadrupole GC-MS.