In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores

• DOI: 10.1007/s11307-015-0897-6
• Publication Date: 2015-09-30T14:53:03Z
• Authors: Milos Petrik; Chuangyan Zhai; Zbynek Novy; Lubor Urbanek; Hubertus Haas; Clemens Decristoforo
• Keywords: Biodistribution; Ex vivo; Affinities

Some [68Ga]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the vitro vivo behaviour selected Ga-68 Zr-89 labelled siderophores. Radiolabelling was performed HEPES or sodium acetate buffer systems. Radiochemical purity siderophores determined using chromatography. Partition coefficients, stability protein binding affinities were determined. Ex biodistribution animal studied mice. Certain differences among observed labelling efficiency. Protein tests showed highest stabilities lowest for [89Zr]triacetylfusarinine C (TAFC). All [89Zr]siderophores exhibited a similar pharmacokinetics mice with exception [89Zr]ferrioxamine E (FOXE). analogous behaviour. Tested could be applied longitudinal positron emission tomography (PET) studies fungal infections especially TAFC development novel bioconjugates.