Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure
Male
Aging
Wound Healing
0303 health sciences
Free Radicals
Antioxidants
Rats
3. Good health
Rats, Sprague-Dawley
Disease Models, Animal
03 medical and health sciences
Ischemia
Animals
Wounds and Injuries
Oxidation-Reduction
Skin
DOI:
10.1007/s11357-014-9617-4
Publication Date:
2014-01-20T01:18:09Z
AUTHORS (7)
ABSTRACT
Advertisements targeted at the elderly population suggest that antioxidant therapy will reduce free radicals and promote wound healing, yet few scientific studies substantiate these claims. To better understand the potential utility of supplemental antioxidant therapy for wound healing, we tested the hypothesis that age and tissue ischemia alter the balance of endogenous antioxidant enzymes. Using a bipedicled skin flap model, ischemic and non-ischemic wounds were created on young and aged rats. Wound closure and the balance of the critical antioxidants superoxide dismutase and glutathione in the wound bed were determined. Ischemia delayed wound closure significantly more in aged rats. Lower superoxide dismutase 2 and glutathione in non-ischemic wounds of aged rats indicate a basal deficit due to age alone. Ischemic wounds from aged rats had lower superoxide dismutase 2 protein and activity initially, coupled with decreased ratios of reduced/oxidized glutathione and lower glutathione peroxidase activity. De novo glutathione synthesis, to restore redox balance in aged ischemic wounds, was initiated as evidenced by increased glutamate cysteine ligase. Results demonstrate deficiencies in two antioxidant pathways in aged rats that become exaggerated in ischemic tissue, culminating in profoundly impaired wound healing and prolonged inflammation.
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