Rapid measurement of ageing by automated monitoring of movement of C. elegans populations

• DOI: 10.1007/s11357-023-00998-w
• Publication Date: 2023-11-08T18:02:39Z
• Authors: Giulia Zavagno; Adelaide Raimundo; Andy Kirby; Christopher Saunter; David Weinkove
• Keywords: 2. Zero hunger; Original Article; 3. Good health

Finding new interventions that slow ageing and maintain human health is a huge challenge of our time. The nematode Caenorhabditis elegans offers rapid in vivo method to determine whether compound extends its 2 3-week lifespan. Measuring lifespan the standard monitor ageing, but will not necessarily health. Here, we describe automated monitoring C. movement from early mid-adulthood as faster healthspan-based measure ageing. Using WormGazer™ technology, multiple Petri dishes each containing several worms are imaged simultaneously non-invasively by an array cameras can be scaled easily. This approach demonstrates most functional decline occurs during first week adulthood. We find 7 days imaging sufficient dose-dependent efficacy sulfamethoxazole compared 40 required for parallel experiment. Understanding any negative consequences important. show long-lived mutant age-1(hx546) stays active longer than wild type it moves slower Thus, continuous analysis rapidly identify while revealing effects on