Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells
Skin, Artificial
0301 basic medicine
Tissue Engineering
Transplantation, Heterologous
Mice, Nude
Bone Marrow Cells
Cell Differentiation
Mesenchymal Stem Cells
Skin Transplantation
Mesenchymal Stem Cell Transplantation
Transplantation, Autologous
3. Good health
Mice
03 medical and health sciences
Microscopy, Electron, Transmission
Animals
Humans
Safety
DOI:
10.1007/s11427-007-0069-2
Publication Date:
2007-07-25T20:14:01Z
AUTHORS (10)
ABSTRACT
To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesenchymal stem cells (BMSCs) were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissueengineered skin. The results showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle shape to round or oval; tonofibrils, melanosomes and keratohyaline granules were observed under a transmission electronic microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% +/- 4.2%) and epidermal cells differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06 pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material shortened skin wound repair significantly. In one word, autogenic BMSCs have the potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering.
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CITATIONS (21)
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