pyrG is required for maintaining stable cellular uracil level and normal sporulation pattern under excess uracil stress in Aspergillus nidulans

• DOI: 10.1007/s11427-013-4480-6
• Publication Date: 2013-04-30T22:30:47Z
• Authors: XianYun Sun; JuFen Zhu; Li Bao; ChengCheng Hu; Cheng Jin; Steven D. Harris; HongWei Liu; ShaoJie Li
• Keywords: Uracil; Aspergillus nidulans

Tight control of the intracellular uracil level is believed to be important reduce occurrence incorporation into DNA. The pyrG gene Aspergillus nidulans encodes orotidine 5'-phosphate decarboxylase, which catalyzes conversion monophosphate (OMP) uridine (UMP). In this study, we found that critical for maintaining at a low concentration in A. cells presence exogenous uracil. Excess and its derivatives had stronger inhibitory effect on growth pyrG89 mutant with defective OMP decarboxylase activity than wild type, induced sexual development but not type. Analysis transcriptomic responses excess by digital expression profiling (DGE) revealed genes related were transcriptionally activated Quantitative analysis HPLC showed cellular was 6.5 times higher type This study only provides new information recycling adaptation also reveals potential effects fungal development.