Mining of a streptothricin gene cluster from Streptomyces sp. TP-A0356 genome via heterologous expression

• DOI: 10.1007/s11427-013-4504-2
• Publication Date: 2013-07-05T04:50:43Z
• Authors: JinE Li; ZhengYan Guo; Wei Huang; XiangXi Meng; GuoMin Ai; GongLi Tang; YiHua Chen
• Keywords: Gene cluster; Heterologous expression; Heterologous

Streptothricins (STs) are used commercially to treat bacterial and fungal diseases in agriculture. Mining of the sequenced microbial genomes uncovered two cryptic ST clusters from Streptomyces sp. C TP-A0356. The cluster S. TP-A0356 was verified by successful heterologous expression coelicolor M145. Two new analogs were produced together with streptothricin F D host. further confirmed inactivation gene stnO, which proposed encoding an aminomutase supplying β-lysines for poly-β-Lys chain formation. A putative biosynthetic pathway STs is based on bioinformatics analyses genes experimental evidence.