Platelet-mediated adhesion facilitates leukocyte sequestration in hypoxia-reoxygenated microvessels

• DOI: 10.1007/s11427-015-4986-1
• Publication Date: 2016-01-18T15:01:59Z
• Authors: Senfeng Zheng; Yanting Cao; Wenjian Zhang; Honglin Liu; Jia You; Yiqing Yin; Jinning Lou; Chenghui Li
• Keywords: Hypoxia

Leukocyte transendothelial migration and sequestration are two distinct outcomes following leukocyte adhesion to endothelium during ischemia-reperfusion injury, in which platelets may play a pivotal role. In the present study, we established an vitro hypoxia-reoxygenation model mimic injury found platelet pre-incubation significantly increased endothelial cells after hyoxia-reoxygenation (over 67%). Blockade of endothelial-cell-expressed molecules inhibited direct cells, while platelet-mediated was suppressed by blockade platelet-expressed molecules. Further experiments revealed acted as bridge mediate adhesion, predominant pattern presence platelets. However, 31%), could be aggravated molecules, but alleviated platelet- expressed This would indicate that disrupted migration. An vivo mesenteric demonstrated transfusion alone caused mild reperfused vessels subsequent infiltration, simultaneous led massive within microvessels. Our studies enhanced Overall, this leads hypoxia-reoxygenated