Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman’s syndrome

• DOI: 10.1007/s11427-016-0328-y
• Publication Date: 2016-12-05T07:50:15Z
• Authors: Guangfeng Zhao; Yun Cao; Xianghong Zhu; Xiaoqiu Tang; Lijun Ding; Haixiang Sun; Juan Li; Xinan Li; Chenyan Dai; Tong Ru; Hui Zhu; Jingjie Lu; Caimei Lin; Jingmei Wang; Guijun Yan; Huiyan Wang; Lei Wang; Yimin Dai; Bin Wang; Ruotian Li; Jianwu Dai; Yan Zhou; Yali Hu
• Keywords: 0303 health sciences; Agricultural and Biological Sciences(all); Tissue Scaffolds; Biochemistry, Genetics and Molecular Biology(all); Cell Transplantation; Tumor Suppressor Proteins; Down-Regulation; Bone Marrow Cells; Gynatresia; Real-Time Polymerase Chain Reaction; 3. Good health; Endometrium; 03 medical and health sciences; Environmental Science(all); Humans; Female; Collagen; Transcription Factors

Asherman's syndrome (AS) is a common disease that presents endometrial regeneration disorder. However, little known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning for patients with AS. Here, we report ΔNp63 significantly upregulated residual epithelial cells impaired AS; upregulated-ΔNp63 induces quiescence alteration stemness. Importantly, demonstrate engrafting high density autologous bone marrow mononuclear (BMNCs) loaded collagen scaffold onto uterine lining downregulates expression, reverses ΔNp63-induced pathological changes, normalizes stemness alterations restores regeneration. Finally, five achieved successful pregnancies live births. Therefore, conclude crucial therapeutic target This novel treatment improves outcome severe