Optimization of evanescent wave imaging for the visualization of protein adsorption layers

• DOI: 10.1007/s11433-010-4102-2
• Publication Date: 2010-08-28T04:23:32Z
• Authors: Li Liu; Yu Niu; She Chen; YongHong Meng; HongWei Ma; Gang Jin
• Keywords: 01 natural sciences; 0104 chemical sciences

We have optimized the settings of evanescent wave imaging for the visualization of a protein adsorption layer. The enhancement of the evanescent wave at the interface brought by the incident angle, the polarized state of light beam as well as a gold layer is considered. In order to improve the image contrast of a protein monolayer in experiments, we have optimized three factors—the incident angle, the polarization of light beam, and the thickness of an introduced thin gold layer with a theoretical simulation.