Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ

• DOI: 10.1007/s11626-022-00699-2
• Publication Date: 2022-09-20T14:06:49Z
• Authors: Langhai Xu; Zhonggai Chen; Tingting Geng; Bin Ru; Quan Wan; Jianbin Zhang; Shun Li; Wenjun Cai
• Keywords: Myokine

Abstract Tendinopathy is a common tendon disorder characterized by pain, swelling, and dysfunction. Current evidence has demonstrated that the depletion of stem cell pool non-tenogenic differentiation tendon-derived stem/progenitor cells (TSPCs) might account for pathogenesis tendinopathy. FNDC5/Irisin, as novel exercise-induced myokine, proved to be involved in protective effects on musculoskeletal disorders. However, whether irisin can affect TSPCs fate still unknown. To ascertain roles proliferation tenogenic TSPCs, rat were isolated incubated with irisin. Cell viability, phenotypic changes, related signaling pathways evaluated CCK-8 assay, colony formation real-time PCR, Western blot, immunofluorescence, proteasome activity assay. We found treatment increased proliferative colony-forming abilities, promoted upregulating expression YAP/TAZ. In conclusion, our work showed first time promotes vitro activating YAP/TAZ, process was associated ubiquitin-proteasome proteolytic pathway. agents targeting YAP/TAZ may promising therapeutic options