Ginsenoside Rg1 relieves rat intervertebral disc degeneration and inhibits IL-1β-induced nucleus pulposus cell apoptosis and inflammation via NF-κB signaling pathway

• DOI: 10.1007/s11626-024-00883-6
• Publication Date: 2024-03-14T17:02:03Z
• Authors: Lei Yu; Ying-Jie Hao; Zhi-Nan Ren; Guang-Duo Zhu; Wei-Wei Zhou; Xu Lian; Xue-Jian Wu
• Keywords: Aggrecan; Intervertebral Disc; MMP3; Ginsenoside Rg1; Intraperitoneal injection; Matrix Metalloproteinase 3

Abstract The study aimed to investigate the effect of ginsenoside Rg1 on intervertebral disc degeneration (IVDD) in rats and IL-1β-induced nucleus pulposus (NP) cells, explore its underlying mechanism. Forty IVDD rat models were divided into group, low-dose (L-Rg1) group (intraperitoneal injection 20 mg/kg/d Rg1), medium-dose (M-Rg1) 40 high-dose (H-Rg1) 80 Rg1). pathological change was observed by HE safranin O-fast green staining. expression IL-1β, IL-6, TNF-α, MMP3, aggrecan, collagen II detected. NF-κB p65 IVD tissues Rat NP cells induced IL-1β simulate environment control 20, 50, 100 µmol/L groups. cell proliferation activity, apoptosis, II, pathway–related protein In rats, improved pathology tissues; suppressed II; inhibited p-p65/p65 nuclear translocation p65, alleviate progression. also apoptosis p-p65/p65, IκK a dose-dependent manner. Ginsenoside alleviated inflammatory response, ECM degradation cells. And may exert via inhibiting activation signaling pathway.