Molecular Investigation of Anaplasma spp. and Genotype Profile of A. ovis in Sheep from Different Farms in Türkiye

• DOI: 10.1007/s11686-025-01021-2
• Publication Date: 2025-04-10T16:47:31Z
• Authors: Özge Dülek; Çağrı Kandemir; Ecem Su Koçkaya; Ecem Sürgeç; Mervenur Güvendi; Muhammet Karakavuk; Aysu Değirmenci D...; Özlem Günay-Esiyok; Turgay Taşkın; Mert Döşkaya; Cemal Ün; Adnan Yüksel Gürüz; Ahmet Efe Köseoğlu; Sedef Erkunt Alak; Erkan Pehlivan; H. Deniz Şireli; Serdar Koçak; Ömer Faruk Yılmaz; Hüseyin Can
• Keywords: Ovis; Anaplasmosis; Tick-borne disease; Parasitology

Abstract Purpose Anaplasmosis is a tick-borne disease which caused by different Anaplasma species. Among species, A. ovis can infect sheep and goats cause ovine anaplasmosis. In this study, the prevalence of spp. was investigated in 31 breeds from regions Türkiye. Method PCR targeting MSP-4 gene blood samples (n = 366) collected Also, some positive were sequenced for species identification sub-cluster analyses. Results The molecular 43.9% (161/366). Anatolian Merino 10) Akkaraman 11) breeds, reached to 100%. highest detected Black Sea region 70% (28/40) lowest Marmara 32% (16/50). While 59.7% produced extensive system, it found as 39.2% 9.8% semi-extensive intensive systems, respectively. Accordingly, these findings suggest that production system protects them diseases, are great economic importance. According BLAST results, all 29) identified . mixed infections 6 samples. phylogenetic tree constructed 38 sequence data showed presence three sub-clusters (Sub-cluster 1, 2, 3). Sub-cluster 2 most prevalent with 42.1% frequency compared other sub-clusters. Conclusion This study grown Türkiye have high value