Liver and intestinal fatty acid‐binding protein expression increases phospholipid content and alters phospholipid fatty acid composition in L‐cell fibroblasts

• DOI: 10.1007/s11745-000-0579-x
• Publication Date: 2006-11-15T13:49:54Z
• Authors: Eric J. Murphy; Daniel R. Prows; Thomas Stiles; Friedhelm Schroeder
• Keywords: Glycerophospholipids; Phosphatidylethanolamine; Plasmalogen; Phospholipid transfer protein; Choline

Although fatty acid-binding proteins (FABP) differentially affect acid uptake, nothing is known regarding their role(s) in determining cellular phospholipid levels and composition. The effects of liver (L)- intestinal (I)-FABP expression on these parameters were determined using stably transfected L-cells. Expression L- I-FABP increased total mass (nmol/mg protein) 1.7- 1.3-fold relative to controls, respectively. L-FABP the masses choline glycerophospholipids (ChoGpl) 1.5-fold, phosphatidylserine (PtdSer) 5.6-fold, ethanolamine 1.4-fold, sphingomyelin 1.7-fold, phosphatidylinositol 2.6-fold. In contrast, only ChoGpl PtdSer, 1.2- 3.1-fold, Surprisingly, both plasmalogen 1.6- 1.1-fold, respectively, while was 2.3- increase resulted dramatic 48 33% decreases cholesterol-to-phospholipid ratio expressing cells, generally polyunsaturated acids, primarily by increasing 20:4n-6 22:6n-3, decreasing 18:1n-9 16:1n-7. proportions. Hence, I- affected mass, class composition, acyl chain enhanced synthesis, effect much greater, consistent with previous work suggesting that two FABP lipid metabolism.