Isolation and Characterization of a Stress‐Dependent Plastidial Δ12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7

• DOI: 10.1007/s11745-009-3381-8
• Publication Date: 2010-01-19T17:14:13Z
• Authors: Yandu Lu; Xiaoyuan Chi; Zhaoxin Li; Qingli Yang; Fuchao Li; Shaofang Liu; Qinhua Gan; Song Qin
• Keywords: Chlorella vulgaris

Abstract An acclimation to the changing physicochemical conditions and high amount of Δ 12 ‐unsaturated fatty acids Antarctic Chlorella vulgaris NJ‐7 prompted us speculate about involvement ‐fatty acid desaturases (FAD) in its adaptation extremely unfavorable ambience. A full‐length cDNA sequence, designated CvFAD6, was isolated from C. via RT‐PCR RACE methods. Sequence alignment showed that gene homologous corresponding ‐FAD other eukaryotes. Phylogenetic analysis it grouped with plastidial conserved histidine boxes. Yeast cells transformed a plasmid construct containing CvFAD6 coding region accumulated considerable linoleic (18:2Δ 9,12 ), normally not present wild‐type yeast cells, suggesting encodes functional enzyme. The correlation between accumulation temperature has been examined by real time PCR. constant expression 25 15 °C whereas fourfold increased 4 °C. Moreover, transcription more sensitive saline stress since 20‐fold increase at 6% NaCl detected. Our data demonstrate is enzyme responsible for desaturation involved low salinity NJ‐7.