Protein Tyrosine Phosphatase‐1B (PTP‐1B) Knockdown Improves Palmitate‐Induced Insulin Resistance in C2C12 Skeletal Muscle Cells

• DOI: 10.1007/s11745-010-3394-3
• Publication Date: 2010-02-22T10:41:27Z
• Authors: Salar Bakhtiyari; Reza Meshkani; Mohammad Taghikhani; Bagher Larijani; Khosrow Adeli
• Keywords: C2C12

Insulin resistance is the central defect in type 2 diabetes and obesity. During development of insulin a lipid accumulation accompanied by increased PTP-1B expression muscle. The aim this study was to examine effects knockdown on signaling presence or absence palmitate C2C12 skeletal muscle cells. A stable cell line established using short hairpin RNA (shRNA) protein PTP1B. Analysis phosphorylation levels IRS-1 Akt were detected western blot. glucose uptake also measured harboring shRNA showed 62% decrease levels. 0.5 mM significantly induced both control (26%) cells (16.5%) compared untreated Under treatment with palmitate, stimulated (Tyr632) (Ser473) 1.55- 1.86-fold, respectively, greater than controls. In dependent about 3-fold higher Our data that decreasing level can enhance activity important elements signaling. improvement action persisted even treated resistant myotubes.