Potential of Magnetic Resonance Spectroscopy in Assessing the Effect of Fatty Acids on Inflammatory Bowel Disease in an Animal Model

• DOI: 10.1007/s11745-010-3455-7
• Publication Date: 2010-08-18T08:00:24Z
• Authors: Sonal Varma; Michael N. A. Eskin; Ranjana Bird; Brion Dolenko; Jayadev Raju; Omkar B. Ijare; Tedros Bezabeh
• Keywords: Lipidology; Animal model

People with inflammatory bowel disease (IBD) are at risk for developing colorectal cancer, and this increases a rate of 1% per year after 8-10 years having the disease. Saturated omega-6 polyunsaturated fatty acids (PUFAs) have been implicated in its causation. Conversely, omega-3 PUFAs may potential to confer therapeutic benefit. Since proton magnetic resonance spectroscopy ((1)H MRS) combined pattern recognition methods could be valuable adjunct histology, objective study was analyze (1)H MRS assessing effect dietary on colonic inflammation. Forty male Sprague-Dawley rats were administered one following regimens 2 weeks: low-fat corn oil (omega-6), high-fat flaxseed (omega-3) or beef tallow (saturated acids). Half animals fed 2% carrageenan induce inflammation similar IBD. histology performed ex vivo samples, MR spectra analyzed using statistical classification strategy (SCS). The histological and/or studies revealed that different modulate differently, being most least inflammatory. is capable identifying biochemical changes tissue as result inflammation, when SCS, technique accurately differentiated inflamed mucosa based severity This indicates serve Our data also suggest both type amount diet critical modulating